Coding

Part:BBa_K2926054:Design

Designed by: Johanna Opgenoorth   Group: iGEM19_Bielefeld-CeBiTec   (2019-10-15)


Fusion protein of mating factor alpha from yeast, mCherry and pVIII from the M13 bacteriophage


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To enable proper protein folding of every subdomain of the fusion protein we designed a short glycine-serine linker sequence and inserted it in between every subdomain.


Source

Mating factor alpha was obtained via oligo synthesis while mCherry was isolated from the iGEM part BBa_J06504 and the phage protein pVIII was excised from a helperphage plasmid purchased from NEB.

References